# Stage 11 — Master Figures Report
**Model:** ASMD/Xenpozyme QSP v2.0
**Style:** 300 DPI PNG, DejaVu Sans (Times/Calibri-equivalent), top/right spines removed for clean look.

## Main figures (4)
| File | Caption |
|---|---|
| `Figure1_Architecture.png` | Five-layer schematic of v2.0: PK three-compartment → ADA modulation → substrate (lysosomal SM) → biomarkers (LysoSM, ceramide) and tissue burden (spleen, liver, lung) → clinical observables (volumes, DLCO, platelet, height-Z). Legend lists 11 species, 53 parameters, 27 dosing events; libSBML L3v2 with 0 errors. |
| `Figure2_Calibration.png` | Six-panel predicted-vs-observed time-courses (W0–W104) for spleen, liver, DLCO, platelet, LysoSM, ceramide; adult (steelblue) and paediatric (orange) overlaid; reliability-A anchors as filled circles with SE bars; reliability-B interpolated points carried only as model line. Demonstrates 96 % calibration / 83 % holdout PASS rate. |
| `Figure3_BiomarkerTrajectories.png` | Six-panel mechanistic trajectories: A. PK three-compartment (log scale); B. ADA titre development; C. Lysosomal SM degradation (% baseline); D. Plasma LysoSM with W52 anchors; E. Plasma ceramide; F. Tissue burden across organs. Shows the cascading mechanism from PK through enzymatic degradation to peripheral biomarkers. |
| `Figure4_Combination_Outcomes.png` | Eight-panel barchart comparing W104 outcomes for four regimens (R1 label, R2 reduced, R3 escalated, R4 ADA(+) sub-population) across all endpoints plus composite efficacy score. Adult and paediatric side-by-side. Demonstrates regimen-saturation: at label dose the model is already at the saturation cap of the dominant parameters. |

## Supplementary figures (10)
| File | Source stage |
|---|---|
| `FigureS1_GSA_heatmap.png` | Stage 7 — full Spearman ρ heatmap, both populations |
| `FigureS2_GSA_barchart.png` | Stage 7 — top-5 dominant parameters per endpoint |
| `FigureS3_RSE_adult.png` | Stage 8 — RSE bar plot, adult |
| `FigureS4_RSE_paediatric.png` | Stage 8 — RSE bar plot, paediatric |
| `FigureS5_FIM_corr_adult.png` | Stage 8 — FIM correlation heatmap, adult |
| `FigureS6_FIM_corr_paediatric.png` | Stage 8 — FIM correlation heatmap, paediatric |
| `FigureS7_PL_adult.png` | Stage 8 — profile likelihood for 5 dominant adult parameters |
| `FigureS8_PL_paediatric.png` | Stage 8 — profile likelihood for 3 dominant paediatric parameters |
| `FigureS9_Forest_plots.png` | Stage 9 — Bayesian forest plots, all endpoints |
| `FigureS10_Posteriors.png` | Stage 9 — pooled posterior distributions |

**Stage 11 status: COMPLETE — proceed to Stage 12 regulatory package.**
